Optimization of culture medium for a Bradyrhizobium japonicum strain introduced from Russia
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چکیده
منابع مشابه
A Selective Medium for the Isolation and Quantification of Bradyrhizobium japonicum and Bradyrhizobium elkanii Strains from Soils and Inoculants.
The ecological examination of members of the family Rhizobiaceae has been hampered by the lack of a selective medium for isolation of root nodule bacteria from soil. A novel non-antibiotic-containing medium has been developed which allows selective isolation of Bradyrhizobium japonicum and B. elkanii strains from soil and inoculants. The medium, BJSM, is based on the resistance of B.japonicum a...
متن کاملOptimization of Culture Conditions and Medium Components for Carboxymethyl Cellulase (CMCase) Production by a Novel Basidiomycete Strain Peniophora sp. NDVN01
Background: Cellulytic enzymes including carboxymethyl cellulases play the key role in hydrolysis of cellulose, a huge organic carbon reservoir on earth, into monomeric sugars and their eventual conversion into valuable chemicals and energy sources. Objectives: In this study, we described the identification of a basidiomycete isolate NDVN01 and optimization of culture conditions and medium com...
متن کاملIsolation of a cytochrome aa(3) gene from Bradyrhizobium japonicum.
Bradyhizobium japonicum strain LO501 is a Tn5-induced mutant that does not express the terminal oxidase cytochrome aa(3) (cytochrome-c oxidase, EC 1.9.3.1). Two and one-half kilobase pairs of LO501 genomic DNA that flanks the transposon was isolated and used as a hybridization probe to obtain the wild-type gene from a cosmid library. Two subcloned fragments from two of the isolated cosmids were...
متن کاملGene Probe Designing for Evaluation of the Diversity of Bradyrhizobium japonicum Isolates
Many researchers consider the use of different probes for hybridization assays as suitable for studying the genetic diversity of nitrogen fixing bacteria. In this study for asessing genetic diversity among Bradyrhizobium japonicum isolates, two different probes (sucA and topA) chosen from the chromosomal genome of Bradyrhizobium strain USDA 110 were designed, evaluated by DNAMAN software and im...
متن کاملIntra- and inter-specific competition in Rhizobium fredii and Bradyrhizobium japonicum as indigenous and introduced organisms
Schmidt 1973; Kapusta and Rouwenhorst 1973; Weaver and Frederick 1974). However, the numbers needed to overcome indigenous rhizobia are in many cases too excessive to be practical. In much of the soybean-growing area in the north central United States, an area encompassing many soil types, indigeneous strains of Bradyrhizobium japonicum serogroup 123 dominate nodulation of soybeans (Damirgi et ...
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ژورنال
عنوان ژورنال: Plant Breeding and Seed Production
سال: 2015
ISSN: 2413-7510,1026-9959
DOI: 10.30835/2413-7510.2015.57402